Hotspot

atpA-atpF

LSC · 11798-11857 · 60 bp

Back to Omphalogramma

Pi 0.03

MarkerSeek score 71.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.03
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TTCGTCGGCTCGAATAGTTG	TTACGTACCATCAGTGCCAA	50.0	45.0	58.1	56.9	140-147 bp	1.0	74.6
atpA-atpF_p2	TTCGTCGGCTCGAATAGTTG	TTTACGTACCATCAGTGCCA	50.0	45.0	58.1	56.9	141-148 bp	1.0	74.6
atpA-atpF_p3	TTCGTCGGCTCGAATAGTTG	ACGTACCATCAGTGCCAATA	50.0	45.0	58.1	56.6	138-145 bp	1.0	72.8
atpA-atpF_p4	TGCTCAATGCGTTCACGTAT	TTACGTACCATCAGTGCCAA	45.0	45.0	58.3	56.9	172-179 bp	1.0	72.3
atpA-atpF_p5	TGCTCAATGCGTTCACGTAT	TTTACGTACCATCAGTGCCA	45.0	45.0	58.3	56.9	173-180 bp	1.0	72.3