Hotspot

matK

LSC · 2072-3592 · 1521 bp

Back to Omalotheca

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACATCAACATTTCAGAATAGAAA	ACCCCCAAAATCCTCTACCT	26.1	50.0	52.4	57.6	1681 bp	1.0	40.0
matK_p2	ACATCAACATTTCAGAATAGAAAT	ACCCCCAAAATCCTCTACCT	25.0	50.0	53.0	57.6	1681 bp	1.0	40.0
matK_p3	ACATCAACATTTCAGAATAGAAA	AACCCCCAAAATCCTCTACC	26.1	50.0	52.4	56.8	1682 bp	1.0	40.0
matK_p4	TACATCAACATTTCAGAATAGAAA	ACCCCCAAAATCCTCTACCT	25.0	50.0	52.5	57.6	1682 bp	1.0	40.0
matK_p5	ACATCAACATTTCAGAATAGAAA	ACCCCCAAAATCCTCTACCTT	26.1	47.6	52.4	58.3	1681 bp	1.0	40.0