Hotspot

trnL-UAG-rpl32

SSC · 122731-123470 · 740 bp

Back to Omalotheca

Pi 0
MarkerSeek score 59.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
740
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 852-858 bp 1.0 79.4
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 853-859 bp 1.0 79.4
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 863-869 bp 1.0 79.3
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 864-870 bp 1.0 79.3
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 862-868 bp 1.0 78.5