Hotspot

ndhE-psaC

SSC · 119202-119457 · 256 bp

Back to Omalotheca

Pi 0

MarkerSeek score 33.80

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 256
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	CTGAAGCCGCTATTGGACTC	ACATCCGTCCCAAGGTATCA	55.0	50.0	58.4	58.1	450 bp	1.0	75.6
ndhE-psaC_p2	CCGCTATTGGACTCGCTATT	ACATCCGTCCCAAGGTATCA	50.0	50.0	57.6	58.1	444 bp	1.0	75.5
ndhE-psaC_p3	CTGAAGCCGCTATTGGACTC	CGGACACATTGAGTACACCC	55.0	55.0	58.4	58.3	404 bp	1.0	74.4
ndhE-psaC_p4	CTGAAGCCGCTATTGGACTC	GGACACATTGAGTACACCCG	55.0	55.0	58.4	58.3	403 bp	1.0	74.4
ndhE-psaC_p5	CCGCTATTGGACTCGCTATT	GGACACATTGAGTACACCCG	50.0	55.0	57.6	58.3	397 bp	1.0	74.3