Hotspot

psbH-petB

LSC · 74732-74855 · 124 bp

Back to Omalotheca

Pi 0.00806

MarkerSeek score 57.10

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 1
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GCAATGGCTTTATTTGCGGT	TCCGTGTTCCAGATACTAGA	45.0	45.0	58.0	54.7	326 bp	1.0	53.9
psbH-petB_p2	TCGCAATGGCTTTATTTGCG	TCCGTGTTCCAGATACTAGA	45.0	45.0	58.1	54.7	328 bp	1.0	53.2
psbH-petB_p3	GCAATGGCTTTATTTGCGGT	TCCGTGTTCCAGATACTAGAA	45.0	42.9	58.0	55.6	326 bp	1.0	52.6
psbH-petB_p4	GCAATGGCTTTATTTGCGGT	TTCCGTGTTCCAGATACTAGA	45.0	42.9	58.0	55.6	327 bp	1.0	52.6
psbH-petB_p5	TCGCAATGGCTTTATTTGCG	TCCGTGTTCCAGATACTAGAA	45.0	42.9	58.1	55.6	328 bp	1.0	51.9