Hotspot

trnG-UCC-trnT-GGU

LSC · 30610-30985 · 376 bp

Back to Omalotheca

Pi 0.00266
MarkerSeek score 55.60
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
376
variable_sites
1
pi
0.00266
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 AATCAAACCGAAAGACCCCT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.0 58.3 499 bp 1.0 70.3
trnG-UCC-trnT-GGU_p2 ATCAAACCGAAAGACCCCTT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.0 58.3 498 bp 1.0 70.3
trnG-UCC-trnT-GGU_p3 ATCAAACCGAAAGACCCCTT AGGGCTTACGCTTTACACTC 45.0 50.0 57.0 57.6 488 bp 1.0 69.3
trnG-UCC-trnT-GGU_p4 ATCAAACCGAAAGACCCCTT GAGGGCTTACGCTTTACACT 45.0 50.0 57.0 57.6 489 bp 1.0 69.3
trnG-UCC-trnT-GGU_p5 ATCAAACCGAAAGACCCCTT GGGCTTACGCTTTACACTCT 45.0 50.0 57.0 57.6 487 bp 1.0 69.3