Hotspot

rps16-trnQ-UUG

LSC · 6284-7292 · 1009 bp

Back to Omalotheca

Pi 0
MarkerSeek score 58.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1009
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1081-1082 bp 1.0 78.4
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1092-1093 bp 1.0 77.8
rps16-trnQ-UUG_p3 GTCGAGTCAAGAGCACCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1151-1152 bp 1.0 77.1
rps16-trnQ-UUG_p4 CACACGGGATCATGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1101-1102 bp 1.0 76.7
rps16-trnQ-UUG_p5 CGAGTCAAGAGCACCTTCAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1149-1150 bp 1.0 76.4