Hotspot

rpl32-trnL-UAG

SSC · 108479-109181 · 703 bp

Back to Oligostachyum

Pi 0.00316
MarkerSeek score 48.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
703
variable_sites
8
pi
0.00316
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-776 bp 1.0 78.9
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 773-775 bp 1.0 78.9
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-769 bp 1.0 78.4
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 766-768 bp 1.0 78.4
rpl32-trnL-UAG_p5 AAGATCATTTTCCAGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 812-813 bp 0.9 74.2