Hotspot

ndhF-rpl32

SSC · 107433-108298 · 866 bp

Back to Oligostachyum

Pi 0.00277
MarkerSeek score 48.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
866
variable_sites
12
pi
0.00277
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 977-1012 bp 1.0 42.4
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 949-984 bp 1.0 42.1
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 950-985 bp 1.0 42.1
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 951-986 bp 1.0 42.1
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 952-987 bp 1.0 42.1