Hotspot

trnC-GCA-rpoB

LSC · 20517-21704 · 1188 bp

Back to Oligostachyum

Pi 0.00149
MarkerSeek score 55.40
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1188
variable_sites
8
pi
0.00149
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1262-1272 bp 1.0 78.0
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1261-1271 bp 1.0 78.0
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGCCTT 50.0 45.0 58.1 57.6 1326-1336 bp 1.0 77.5
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG AGTTCTTCTGCCAAGCCTTG 50.0 50.0 58.1 58.4 1325-1335 bp 1.0 77.4
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CAAGTTCTTCTGCCAAGCCT 50.0 50.0 58.1 58.4 1327-1337 bp 1.0 77.4