Hotspot

petN-trnC-GCA

LSC · 19548-20445 · 898 bp

Back to Oligostachyum

Pi 0.00129

MarkerSeek score 46.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 5
pi: 0.00129
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	947-959 bp	1.0	70.8
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	949-961 bp	1.0	70.3
petN-trnC-GCA_p3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	1003-1015 bp	1.0	69.7
petN-trnC-GCA_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.3	59.1	1005-1017 bp	1.0	69.3
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.4	58.1	1004-1016 bp	1.0	69.0