Hotspot

trnG-UCC-trnT-GGU

LSC · 13013-16359 · 3347 bp

Back to Oligostachyum

Pi 0.00171

MarkerSeek score 50.50

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 3347
variable_sites: 23
pi: 0.00171
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	GAAAACCCATAGCCAGGAGG	AAGGAATTCGCGCTCTTCAT	55.0	45.0	57.9	58.0	403-409 bp	1.0	81.4
trnG-UCC-trnT-GGU_p2	CTTAAACGGAGAAGCGGACA	AAGGAATTCGCGCTCTTCAT	50.0	45.0	57.9	58.0	756-762 bp	1.0	80.5
trnG-UCC-trnT-GGU_p3	TTAAACGGAGAAGCGGACAG	AAGGAATTCGCGCTCTTCAT	50.0	45.0	57.9	58.0	755-761 bp	1.0	80.5
trnG-UCC-trnT-GGU_p4	ACTTAAACGGAGAAGCGGAC	AAGGAATTCGCGCTCTTCAT	50.0	45.0	57.9	58.0	757-763 bp	1.0	80.5
trnG-UCC-trnT-GGU_p5	TCTGGTTAGTCGATCCTCCC	AAGGAATTCGCGCTCTTCAT	55.0	45.0	57.9	58.0	699 bp	0.9	75.8