Hotspot

rps2

LSC · 23039-23749 · 711 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TTTTTCAAAAGGGGGAGGCA	TCTATGAAGGTTCCGAAATGA	45.0	38.1	57.8	54.4	789 bp	1.0	41.5
rps2_p2	TTTTTCAAAAGGGGGAGGCA	TCGATAAATCTATGAAGGTTCCGA	45.0	37.5	57.8	57.4	797 bp	1.0	41.4
rps2_p3	TTTTCAAAAGGGGGAGGCAG	TCTATGAAGGTTCCGAAATGA	50.0	38.1	58.3	54.4	788 bp	1.0	40.7
rps2_p4	TTTTCAAAAGGGGGAGGCAG	TCGATAAATCTATGAAGGTTCCGA	50.0	37.5	58.3	57.4	796 bp	1.0	40.6
rps2_p5	TTTTTCAAAAGGGGGAGGCA	TCTATGAAGGTTCCGAAATGAA	45.0	36.4	57.8	55.2	789 bp	1.0	40.0