Hotspot

matK

LSC · 2029-3549 · 1521 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GTGATCAAATTTTTATTTACAACG	CCCCCAAATCCTCTACCTTTC	25.0	52.4	52.1	57.7	1596 bp	1.0	40.0
matK_p2	GTGATCAAATTTTTATTTACAACG	CCCCCAAATCCTCTACCTTT	25.0	50.0	52.1	56.5	1596 bp	1.0	40.0
matK_p3	GTGATCAAATTTTTATTTACAACG	CCCCAAATCCTCTACCTTTCA	25.0	47.6	52.1	57.0	1595 bp	1.0	40.0
matK_p4	GTGATCAAATTTTTATTTACAACG	CCTTGTTTTGACTGTATCGCA	25.0	42.9	52.1	56.8	1636 bp	1.0	40.0
matK_p5	GTGATCAAATTTTTATTTACAACG	ACCTTGTTTTGACTGTATCGC	25.0	42.9	52.1	56.8	1637 bp	1.0	40.0