Hotspot

trnK-UUU-rps16

LSC · 4321-5099 · 779 bp

Back to Oligochaeta

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
779
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 976 bp 1.0 76.8
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 975 bp 1.0 76.8
trnK-UUU-rps16_p3 TCAGTCGTCGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.9 58.3 1000 bp 1.0 76.5
trnK-UUU-rps16_p4 CAGTCGTCGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.9 58.3 999 bp 1.0 76.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 914 bp 1.0 76.1