Hotspot

trnH-GUG-psbA

LSC · 78-436 · 359 bp

Back to Oligochaeta

Pi 0

MarkerSeek score 35.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TGGAAGTGATGCATGAACGT	55.0	45.0	58.1	57.8	460 bp	1.0	77.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TGGAAGTGATGCATGAACGT	50.0	45.0	57.9	57.8	474 bp	1.0	77.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TGGAAGTGATGCATGAACGT	50.0	45.0	57.9	57.8	473 bp	1.0	77.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TGGAAGTGATGCATGAACGT	50.0	45.0	57.9	57.8	475 bp	1.0	77.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	477 bp	1.0	76.3