Hotspot

ndhF

SSC · 111637-113853 · 2217 bp

Back to Olea

Pi 0.00772
MarkerSeek score 48.60
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2217
variable_sites
67
pi
0.00772
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GCAGAATACCGTCACCCATT GAGGTAAGATCCGGTGAATCG 50.0 52.4 58.0 58.0 2628-2634 bp 1.0 74.0
ndhF_p2 GAGATCCGCCGAAGAAGATC GAGGTAAGATCCGGTGAATCG 55.0 52.4 57.9 58.0 2492-2498 bp 1.0 73.9
ndhF_p3 CAGGAACAAGAGAGATCCGC GAGGTAAGATCCGGTGAATCG 55.0 52.4 58.1 58.0 2503-2509 bp 1.0 73.8
ndhF_p4 CGCCGAAGAAGATCCTTCTC GAGGTAAGATCCGGTGAATCG 55.0 52.4 58.2 58.0 2486-2487 bp 0.956522 71.2
ndhF_p5 GCCGAAGAAGATCCTTCTCC CGCTGTCCCATATCCTTTCC 55.0 55.0 57.8 58.1 2996-3000 bp 0.478261 58.8