Hotspot

rpl32-trnL(UAG)

SSC · 114492-115350 · 859 bp

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Pi 0.0125
MarkerSeek score 57.80
Primer available yes
Label rpl32-trnL(UAG)

Feature Metadata

label_name
rpl32-trnL(UAG)
feature_type
igs
region
SSC
length_bp
859
variable_sites
39
pi
0.0125
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(UAG)_p1 ACCGGGAATTCAAAAAGTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.9 57.9 626-949 bp 1.0 53.4
rpl32-trnL(UAG)_p2 ACCGGGAATTCAAAAAGTTT AGCAGCGTGTCTACCAATTT 35.0 45.0 53.9 57.8 619-942 bp 1.0 53.0
rpl32-trnL(UAG)_p3 ACCGGGAATTCAAAAAGTTT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 53.9 57.8 625-948 bp 1.0 52.8
rpl32-trnL(UAG)_p4 ACCGGGAATTCAAAAAGTTT CTAAGAGCAGCGTGTCTACC 35.0 55.0 53.9 57.8 624-947 bp 1.0 52.8
rpl32-trnL(UAG)_p5 ACCGGGAATTCAAAAAGTTTT TCCTAAGAGCAGCGTGTCTA 33.3 50.0 54.7 57.9 626-949 bp 1.0 52.4