Hotspot

ndhF-rpl32

SSC · 113854-114332 · 479 bp

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Pi 0.017
MarkerSeek score 55.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
479
variable_sites
32
pi
0.017
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA TGATGCAGAAGTACGTTTTT 42.9 35.0 57.9 53.5 549-558 bp 1.0 46.1
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA TTGATGCAGAAGTACGTTTT 42.9 35.0 57.9 53.5 550-559 bp 1.0 46.1
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA TTTGATGCAGAAGTACGTTT 42.9 35.0 57.9 53.5 551-560 bp 1.0 46.0
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA TTTTGATGCAGAAGTACGTT 42.9 35.0 57.9 53.5 552-561 bp 1.0 46.0
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA TTTTTGATGCAGAAGTACGT 42.9 35.0 57.9 53.5 553-562 bp 1.0 46.0