Hotspot

ycf4-cemA

LSC · 62231-63103 · 873 bp

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Pi 0.00956
MarkerSeek score 67.30
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
873
variable_sites
31
pi
0.00956
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG GAGAAATCCACCAGGGCAAA 55.0 50.0 58.0 58.1 711-1071 bp 1.0 85.8
ycf4-cemA_p2 ATTCCCTTGACCCGTACTGA GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 710-1070 bp 1.0 85.6
ycf4-cemA_p3 TTCCCTTGACCCGTACTGAT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 709-1069 bp 1.0 85.6
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 636-996 bp 1.0 85.2
ycf4-cemA_p5 GAACAAAAAGCCGCCGAATT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.2 58.1 665-1025 bp 1.0 84.4