Hotspot

atpB-rbcL

LSC · 56062-56846 · 785 bp

Back to Olea

Pi 0.0118
MarkerSeek score 56.40
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
785
variable_sites
26
pi
0.0118
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 ATACGGCCCAGGTTTTGTTT AACACCCGCTTTGAATCCAA 45.0 45.0 58.0 58.3 903-911 bp 1.0 79.9
atpB-rbcL_p2 GGAAACCCCAGAACCAGAAG AACACCCGCTTTGAATCCAA 55.0 45.0 58.1 58.3 874-882 bp 1.0 79.3
atpB-rbcL_p3 GGCGGAAAGGCTACATCTAG AACACCCGCTTTGAATCCAA 55.0 45.0 57.8 58.3 945-953 bp 1.0 78.7
atpB-rbcL_p4 TACGGCCCAGGTTTTGTTTT AACACCCGCTTTGAATCCAA 45.0 45.0 58.2 58.3 902-910 bp 1.0 78.2
atpB-rbcL_p5 GGACCGATGATTTGGACGAT AACACCCGCTTTGAATCCAA 50.0 45.0 57.8 58.3 921-929 bp 1.0 78.1