Hotspot

ycf3-trnS(GGA)

LSC · 45737-46532 · 796 bp

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Pi 0.00462
MarkerSeek score 56.40
Primer available yes
Label ycf3-trnS(GGA)

Feature Metadata

label_name
ycf3-trnS(GGA)
feature_type
igs
region
LSC
length_bp
796
variable_sites
21
pi
0.00462
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS(GGA)_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 878-899 bp 1.0 75.5
ycf3-trnS(GGA)_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 899-920 bp 1.0 74.9
ycf3-trnS(GGA)_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 849-870 bp 1.0 74.9
ycf3-trnS(GGA)_p4 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 879-900 bp 1.0 73.2
ycf3-trnS(GGA)_p5 TTTATCCGCGATCTAGGCAT CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.6 58.1 876-897 bp 1.0 72.5