Hotspot

atpH-atpI

LSC · 14276-15357 · 1082 bp

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Pi 0.00885
MarkerSeek score 59.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1082
variable_sites
37
pi
0.00885
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACAGAAGCGGCAGAAATCA TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.1 58.3 1203-1216 bp 1.0 80.5
atpH-atpI_p2 TACCCTCTACAGCTTGACCC TCCCTGTCATGTTCCTTGGA 55.0 50.0 58.1 58.3 1288-1301 bp 1.0 80.0
atpH-atpI_p3 GCGATACCCTCTACAGCTTG TCCCTGTCATGTTCCTTGGA 55.0 50.0 57.9 58.3 1292-1305 bp 1.0 80.0
atpH-atpI_p4 CAGCAGCAATAACAGAAGCG TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1213-1226 bp 1.0 79.9
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1220-1233 bp 1.0 79.6