Hotspot

atpA-atpF

LSC · 12351-12412 · 62 bp

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Pi 0.0273
MarkerSeek score 56.60
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
62
variable_sites
4
pi
0.0273
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 ATGCCATCGCCTACTTGAAG TGTTGGGAGCGATGAAAGAA 50.0 45.0 58.0 57.4 210-229 bp 1.0 77.4
atpA-atpF_p2 ATGCCATCGCCTACTTGAAG ATGTTGGGAGCGATGAAAGA 50.0 45.0 58.0 57.2 211-230 bp 1.0 75.6
atpA-atpF_p3 ATGCCATCGCCTACTTGAAG CATGTTGGGAGCGATGAAAG 50.0 50.0 58.0 57.2 212-231 bp 1.0 75.2
atpA-atpF_p4 CAATGCCATCGCCTACTTGA TGTTGGGAGCGATGAAAGAA 50.0 45.0 58.3 57.4 212-231 bp 1.0 75.1
atpA-atpF_p5 ATGCCATCGCCTACTTGAAG ATATTGGCATGTTGGGAGCG 50.0 50.0 58.0 58.7 219-228 bp 0.956522 74.0