Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9014-9720 · 707 bp

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Pi 0.0153
MarkerSeek score 63.90
Primer available yes
Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name
trnS(GCU)-trnG(GCC)
feature_type
igs
region
LSC
length_bp
707
variable_sites
44
pi
0.0153
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCU)-trnG(GCC)_p1 CAATCCGACGCTTTAGTCCA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 798-831 bp 1.0 68.3
trnS(GCU)-trnG(GCC)_p2 AATCCGACGCTTTAGTCCAC AGACCGAAAGACCCTTTAACT 50.0 42.9 58.0 56.6 797-830 bp 1.0 68.3
trnS(GCU)-trnG(GCC)_p3 ATCAACGGAACCGGAAAGAG AGACCGAAAGACCCTTTAACT 50.0 42.9 57.9 56.6 862-895 bp 1.0 67.0
trnS(GCU)-trnG(GCC)_p4 CATCAACGGAACCGGAAAGA AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 863-896 bp 1.0 66.2
trnS(GCU)-trnG(GCC)_p5 TCATCAACGGAACCGGAAAG AGACCGAAAGACCCTTTAACT 50.0 42.9 58.2 56.6 864-897 bp 1.0 66.2