Hotspot

rps16-trnQ(UUG)

LSC · 6435-7691 · 1257 bp

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Pi 0.00933
MarkerSeek score 57.20
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1257
variable_sites
48
pi
0.00933
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 CGGATCTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1336-1759 bp 1.0 82.1
rps16-trnQ(UUG)_p2 ATCTGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1333-1756 bp 1.0 82.1
rps16-trnQ(UUG)_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1322-1745 bp 1.0 81.7
rps16-trnQ(UUG)_p4 CCACAACGGATCTGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1342-1765 bp 1.0 80.6
rps16-trnQ(UUG)_p5 GGATCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1335-1758 bp 1.0 80.0