Hotspot

rpl16

LSC · 80307-81808 · 1502 bp

Back to Oldeania

Pi 0.00555

MarkerSeek score 50.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1502
variable_sites: 16
pi: 0.00555
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TAACCGCTGCATTGTCATCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2514-2539 bp	1.0	79.1
rpl16_p2	TGATAACCGCTGCATTGTCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2517-2542 bp	1.0	79.1
rpl16_p3	TCAGCAATAGTGTCCTTGCC	GGACTCAGGCTCAAGTTCTG	50.0	55.0	57.9	57.9	2792-2816 bp	1.0	79.0
rpl16_p4	ACCGTCTTCGCATTTGAACT	GGACTCAGGCTCAAGTTCTG	45.0	55.0	58.1	57.9	2480-2505 bp	1.0	79.0
rpl16_p5	TCTCAATTCCTCGGCAATCG	GGACTCAGGCTCAAGTTCTG	50.0	55.0	58.1	57.9	2579 bp	0.25	48.8