Hotspot

rpl32-trnL-UAG

SSC · 108410-109119 · 710 bp

Back to Oldeania

Pi 0.00847
MarkerSeek score 56.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
710
variable_sites
11
pi
0.00847
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 779-782 bp 1.0 79.4
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 778-781 bp 1.0 79.4
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 772-775 bp 1.0 79.0
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 771-774 bp 1.0 79.0
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA GCTTGAGCACTGCTTCCTAA 45.0 50.0 58.1 58.2 792-795 bp 1.0 78.9