Hotspot

trnP-GGG-psaJ

LSC · 66752-67176 · 425 bp

Back to Oldeania

Pi 0.00864

MarkerSeek score 60.80

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 6
pi: 0.00864
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	509-533 bp	1.0	83.0
trnP-GGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	510-534 bp	1.0	82.5
trnP-GGG-psaJ_p3	CCTCGCTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	55.0	45.0	58.1	57.9	585-609 bp	1.0	82.3
trnP-GGG-psaJ_p4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	517-541 bp	1.0	82.3
trnP-GGG-psaJ_p5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	514-538 bp	1.0	80.6