Hotspot

ycf4-cemA

LSC · 60679-61094 · 416 bp

Back to Oldeania

Pi 0.0149

MarkerSeek score 67.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 416
variable_sites: 12
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	508-520 bp	1.0	81.1
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	510-522 bp	1.0	80.8
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	537-549 bp	1.0	80.4
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.7	57.8	513-525 bp	1.0	79.2
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.4	57.8	517-529 bp	1.0	78.9