Hotspot

rbcL-psaI

LSC · 58355-59640 · 1286 bp

Back to Oldeania

Pi 0.00739

MarkerSeek score 64.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 16
pi: 0.00739
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.1	58.0	1395-1422 bp	1.0	80.3
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1425-1452 bp	1.0	79.8
rbcL-psaI_p3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.1	57.9	1401-1428 bp	1.0	79.7
rbcL-psaI_p4	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1396-1423 bp	1.0	79.2
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1431-1458 bp	1.0	79.1