Hotspot

atpB-rbcL

LSC · 56142-56920 · 779 bp

Back to Oldeania

Pi 0.00513

MarkerSeek score 62.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 7
pi: 0.00513
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.2	58.0	973-974 bp	1.0	78.6
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	972-973 bp	1.0	78.6
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	CCTTGGTTTCATACTCCGGG	50.0	55.0	58.2	58.0	970-971 bp	1.0	78.4
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTTGGTTTCATACTCCGGGG	50.0	55.0	58.2	58.0	969-970 bp	1.0	78.4
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	CTTGGTTTCATACTCCGGGG	55.0	55.0	57.7	58.0	987-988 bp	1.0	78.1