Hotspot

trnfM-CAU-trnT-GGU

LSC · 13453-16256 · 2804 bp

Back to Oldeania

Pi 0.00405
MarkerSeek score 47.00
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
2804
variable_sites
22
pi
0.00405
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG TCGAACCGATGACTTACGTC 55.0 50.0 57.8 57.5 2964-2976 bp 0.75 66.4
trnfM-CAU-trnT-GGU_p2 CCTCAAGGTTATGAGCCTCG CGAACCGATGACTTACGTCT 55.0 50.0 57.8 57.5 2963-2975 bp 0.75 66.3
trnfM-CAU-trnT-GGU_p3 CCTCGTGAGCTACCAAACTG TCGAACCGATGACTTACGTC 55.0 50.0 58.3 57.5 2949-2961 bp 0.75 66.0
trnfM-CAU-trnT-GGU_p4 CCTCGTGAGCTACCAAACTG CGAACCGATGACTTACGTCT 55.0 50.0 58.3 57.5 2948-2960 bp 0.75 65.9
trnfM-CAU-trnT-GGU_p5 CAAGGTTATGAGCCTCGTGA TCGAACCGATGACTTACGTC 50.0 50.0 57.3 57.5 2961-2973 bp 0.75 62.9