Hotspot

trnS-GCU-psbD

LSC · 8409-9388 · 980 bp

Back to Oldeania

Pi 0.00732

MarkerSeek score 64.50

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 14
pi: 0.00732
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1125-1145 bp	1.0	79.7
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1112-1132 bp	1.0	79.2
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	50.0	50.0	57.6	58.2	1111-1131 bp	1.0	76.8
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	45.0	55.0	58.0	58.6	1104-1124 bp	1.0	76.6
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.6	58.2	1098-1118 bp	1.0	76.2