Hotspot

ycf2

IRa · 155165-162247 · 7083 bp

Back to Oenothera

Pi 0.00263

MarkerSeek score 35.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 7083
variable_sites: 48
pi: 0.00263
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GCAGGAGCGAAACAATCAAC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	879-1027 bp	1.0	85.5
ycf2_p2	CCCTCCTTTTTCCGATCCAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	987-1135 bp	1.0	85.3
ycf2_p3	AGGAAGATCCAGAAAGGCCT	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	1197-1345 bp	1.0	85.2
ycf2_p4	GAATAGCCGGGACTTTGAGG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	1214-1362 bp	1.0	85.0
ycf2_p5	CCACAGTGGACCTCTCAGTA	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.1	58.0	1365-1513 bp	1.0	84.6