Hotspot

trnIGAU-rrn16

IRa · 145204-146103 · 900 bp

Back to Oenothera

Pi 0.0287

MarkerSeek score 62.90

Primer available yes

Label trnIGAU-rrn16

Feature Metadata

label_name: trnIGAU-rrn16
feature_type: igs
region: IRa
length_bp: 900
variable_sites: 47
pi: 0.0287
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnIGAU-rrn16_p1	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	543-1045 bp	1.0	84.4
trnIGAU-rrn16_p2	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	511-1013 bp	1.0	84.4
trnIGAU-rrn16_p3	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	57.8	57.7	562-1064 bp	1.0	84.3
trnIGAU-rrn16_p4	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	57.8	57.6	552-1054 bp	1.0	83.9
trnIGAU-rrn16_p5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	50.0	50.0	57.8	57.6	571-1073 bp	1.0	83.8