Hotspot

ycf1-ndhF

SSC · 136033-136289 · 257 bp

Back to Oenothera

Pi 0.0274

MarkerSeek score 49.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 20
pi: 0.0274
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AGTGAGCAATGGGAAAGCAA	TACCATAGTAGCGGCATGGA	45.0	50.0	58.0	58.0	1325-1524 bp	1.0	85.6
ycf1-ndhF_p2	TCCGCATTGACTTAGTGAGC	TACCATAGTAGCGGCATGGA	50.0	50.0	58.0	58.0	1338-1537 bp	1.0	85.5
ycf1-ndhF_p3	AGTGAGCAATGGGAAAGCAA	GGCATGGATAAGAGCGGAAA	45.0	50.0	58.0	58.0	1313-1512 bp	1.0	85.4
ycf1-ndhF_p4	TCCAGCCCATTTCAGGACTA	TACCATAGTAGCGGCATGGA	50.0	50.0	58.0	58.0	1529-1728 bp	1.0	85.2
ycf1-ndhF_p5	TGCCGATTCAACCTGTTTCA	TACCATAGTAGCGGCATGGA	45.0	50.0	58.0	58.0	1694-1893 bp	1.0	84.9