Hotspot

petA-psbJ

LSC · 67738-68770 · 1033 bp

Back to Oenothera

Pi 0.00144

MarkerSeek score 51.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1033
variable_sites: 4
pi: 0.00144
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.0	57.8	1143-1272 bp	1.0	82.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	50.0	55.0	58.0	58.3	1094-1223 bp	1.0	81.6
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.8	57.8	1126-1255 bp	1.0	81.2
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.2	57.8	1140-1269 bp	1.0	81.0
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	ATACTCCGGGTTAGGCTCAT	50.0	50.0	58.0	57.6	1095-1224 bp	1.0	80.8