Hotspot

trnQUUG-accD

LSC · 59153-61736 · 2584 bp

Back to Oenothera

Pi 0.072

MarkerSeek score 66.40

Primer available yes

Label trnQUUG-accD

Feature Metadata

label_name: trnQUUG-accD
feature_type: igs
region: LSC
length_bp: 2584
variable_sites: 461
pi: 0.072
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQUUG-accD_p1	GAAATTGAAATGGGGCGTGG	CTTCCGCTTCCTCGTTAGAC	50.0	55.0	58.0	58.1	2323-2867 bp	0.846154	85.1
trnQUUG-accD_p2	CCGTCGATATTTGCACTGGA	CTTCCGCTTCCTCGTTAGAC	50.0	55.0	58.1	58.1	2390-2934 bp	0.846154	84.6
trnQUUG-accD_p3	TCCGTCGATATTTGCACTGG	CTTCCGCTTCCTCGTTAGAC	50.0	55.0	58.1	58.1	2391-2935 bp	0.846154	84.6
trnQUUG-accD_p4	GAGGTTCGAATCCTTCCGTC	CTTCCGCTTCCTCGTTAGAC	55.0	55.0	58.1	58.1	2265-2809 bp	0.846154	84.5
trnQUUG-accD_p5	GAAATTGAAATGGGGCGTGG	TCTTCCGCTTCCTCGTTAGA	50.0	50.0	58.0	58.2	2324-2868 bp	0.846154	84.4