Hotspot

trnYGUA-trnDGUC

LSC · 33859-34344 · 486 bp

Back to Oenothera

Pi 0.00137

MarkerSeek score 46.70

Primer available yes

Label trnYGUA-trnDGUC

Feature Metadata

label_name: trnYGUA-trnDGUC
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnYGUA-trnDGUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	547-573 bp	1.0	76.2
trnYGUA-trnDGUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	546-572 bp	1.0	76.1
trnYGUA-trnDGUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	568-594 bp	1.0	75.2
trnYGUA-trnDGUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	567-593 bp	1.0	75.0
trnYGUA-trnDGUC_p5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	601-627 bp	1.0	74.6