Hotspot

psbD-trnTGGU

LSC · 31912-33339 · 1428 bp

Back to Oenothera

Pi 0.00106

MarkerSeek score 45.00

Primer available yes

Label psbD-trnTGGU

Feature Metadata

label_name: psbD-trnTGGU
feature_type: igs
region: LSC
length_bp: 1428
variable_sites: 5
pi: 0.00106
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnTGGU_p1	GACCGGACCAACCTACAAAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.7	58.1	1558-1592 bp	1.0	79.9
psbD-trnTGGU_p2	GACCGGACCAACCTACAAAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	57.7	58.1	1561-1595 bp	1.0	79.7
psbD-trnTGGU_p3	TAGACCGGACCAACCTACAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.7	58.1	1560-1594 bp	1.0	79.5
psbD-trnTGGU_p4	TAGACCGGACCAACCTACAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	57.7	58.1	1563-1597 bp	1.0	79.3
psbD-trnTGGU_p5	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.3	58.1	1566-1600 bp	1.0	79.2