Hotspot

trnLUAA-trnTUGU

LSC · 16781-17892 · 1112 bp

Back to Oenothera

Pi 0.00176
MarkerSeek score 48.30
Primer available yes
Label trnLUAA-trnTUGU

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnLUAA-trnTUGU_p1 ACCAATTTCGCCATATCCCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.0 57.9 1110-1162 bp 1.0 83.0
trnLUAA-trnTUGU_p2 TAGCGTCTACCAATTTCGCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.1 57.9 1118-1170 bp 1.0 82.4
trnLUAA-trnTUGU_p3 ACCAATTTCGCCATATCCCC GGAATCGAACCGATGACCAT 50.0 50.0 58.0 57.8 1142-1194 bp 1.0 82.0
trnLUAA-trnTUGU_p4 TAGCGTCTACCAATTTCGCC GGAATCGAACCGATGACCAT 50.0 50.0 58.1 57.8 1150-1202 bp 1.0 81.5
trnLUAA-trnTUGU_p5 AGTCCGTAGCGTCTACCAAT GCGATGCTCTAACCTCTGAG 50.0 55.0 58.2 57.9 1124-1176 bp 1.0 81.1