Hotspot

ndhD

SSC · 116346-117848 · 1503 bp

Back to Oenanthe

Pi 0.00621

MarkerSeek score 43.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 25
pi: 0.00621
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTAATGAAGCATGGGGGTCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.0	58.0	2229-2231 bp	1.0	81.6
ndhD_p2	GAAGCATGGGGGTCCTATTG	GATACCCTGGGACGGATGTA	55.0	55.0	58.0	58.0	2224-2226 bp	1.0	81.6
ndhD_p3	CTAATGAAGCATGGGGGTCC	ATTTCTTGAGCGTTCGGGTT	55.0	45.0	58.0	58.1	2131-2133 bp	1.0	80.9
ndhD_p4	GAAGCATGGGGGTCCTATTG	ATTTCTTGAGCGTTCGGGTT	55.0	45.0	58.0	58.1	2126-2128 bp	1.0	80.9
ndhD_p5	GTGAGAACCATGGGAATCCC	GATACCCTGGGACGGATGTA	55.0	55.0	57.9	58.0	1916-1917 bp	0.857143	75.2