Hotspot

rpl32-trnL-UAG

SSC · 114432-114975 · 544 bp

Back to Oenanthe

Pi 0.0128
MarkerSeek score 62.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
544
variable_sites
18
pi
0.0128
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTCTTTCTACCGGAAACTCGA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.2 58.0 602-636 bp 1.0 71.5
rpl32-trnL-UAG_p2 TCTCTTTCTACCGGAAACTCG AGCGTGTCTACCGATTTCAC 47.6 50.0 57.2 58.0 603-637 bp 1.0 71.5
rpl32-trnL-UAG_p3 CTCTTTCTACCGGAAACTCGA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.2 58.0 603-637 bp 1.0 71.5
rpl32-trnL-UAG_p4 TCTCTTTCTACCGGAAACTCG CAGCGTGTCTACCGATTTCA 47.6 50.0 57.2 58.0 604-638 bp 1.0 71.5
rpl32-trnL-UAG_p5 CTCTTTCTACCGGAAACTCGA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.2 57.9 612-646 bp 1.0 70.5