Hotspot

clpP-psbB

LSC · 72262-72713 · 452 bp

Back to Oenanthe

Pi 0.00843

MarkerSeek score 58.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 10
pi: 0.00843
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GCACTATACGTCAACCCAGG	TATGGACAGAAAGCAACCGG	55.0	50.0	58.1	57.9	596-602 bp	1.0	80.1
clpP-psbB_p2	CAGGACTTCGAAAAGGCACT	TATGGACAGAAAGCAACCGG	50.0	50.0	58.1	57.9	561-567 bp	1.0	79.8
clpP-psbB_p3	GTCAACCCAGGATGCATCTT	TATGGACAGAAAGCAACCGG	50.0	50.0	57.9	57.9	587-593 bp	1.0	79.6
clpP-psbB_p4	AGGCACTTTTGGAACACCAA	TATGGACAGAAAGCAACCGG	45.0	50.0	58.1	57.9	548-554 bp	1.0	79.6
clpP-psbB_p5	CGGGGAAATACCGTATGACC	TATGGACAGAAAGCAACCGG	55.0	50.0	57.8	57.9	634-640 bp	1.0	79.2