Hotspot

psbE-petL

LSC · 64997-66212 · 1216 bp

Back to Oenanthe

Pi 0.00703

MarkerSeek score 58.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 24
pi: 0.00703
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	1269-1298 bp	1.0	81.9
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GGTTACCGTTAAAGCCGCTA	55.0	50.0	58.2	58.0	1264-1293 bp	1.0	81.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.8	57.9	1272-1301 bp	1.0	81.3
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TGAGGTTACCGTTAAAGCCG	55.0	50.0	58.2	57.9	1267-1296 bp	1.0	81.0
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GGTTACCGTTAAAGCCGCTA	50.0	50.0	58.4	58.0	1262-1291 bp	1.0	80.4