Hotspot

trnE-UUC-trnT-GGU

LSC · 31358-32226 · 869 bp

Back to Oenanthe

Pi 0.0136

MarkerSeek score 65.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 31
pi: 0.0136
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	909-972 bp	1.0	85.2
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	906-969 bp	1.0	84.1
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	AGCTAAAGCCCCTTATCGGA	50.0	50.0	57.8	58.2	932-995 bp	1.0	83.6
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.8	58.3	911-974 bp	1.0	83.0
trnE-UUC-trnT-GGU_p5	TTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	944-1007 bp	1.0	82.0