Hotspot

atpF-atpH

LSC · 13144-13517 · 374 bp

Back to Oenanthe

Pi 0.028

MarkerSeek score 71.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 25
pi: 0.028
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CGGAGGGAAAAATACGAGGG	45.0	55.0	57.9	58.1	525-530 bp	1.0	85.2
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CGGAGGGAAAAATACGAGGG	50.0	55.0	57.9	58.1	526-531 bp	1.0	84.6
atpF-atpH_p3	TGGCCCAAAGAAACGAAAGA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	57.9	58.2	459-464 bp	1.0	84.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGG	50.0	55.0	57.9	58.1	556-561 bp	1.0	84.4
atpF-atpH_p5	AGTGGCCCAAAGAAACGAAA	CGGAGGGAAAAATACGAGGG	45.0	55.0	58.2	58.1	527-532 bp	1.0	83.8