Hotspot

trnH-GUG-psbA

LSC · 76-255 · 180 bp

Back to Oenanthe

Pi 0.0405

MarkerSeek score 70.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 21
pi: 0.0405
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	ACCGCGCTAATCTTGGTATG	55.0	50.0	58.1	58.1	301-302 bp	1.0	83.7
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CGCGCTAATCTTGGTATGGA	55.0	50.0	58.1	57.9	299-300 bp	1.0	83.7
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	315-316 bp	1.0	83.4
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	314-315 bp	1.0	83.4
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	ACCGCGCTAATCTTGGTATG	50.0	50.0	57.9	58.1	316-317 bp	1.0	83.4