Hotspot

psbI

LSC · 60094-62608 · 2515 bp

Back to Oedogonium

Pi 0.179

MarkerSeek score 81.80

Primer available yes

Label psbI

Feature Metadata

label_name: psbI
feature_type: gene
region: LSC
length_bp: 2515
variable_sites: 711
pi: 0.179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI_p1	CGATATATCGGACCCAATGGG	ACCAAGCAAAGCTTCTACGA	52.4	45.0	57.8	57.5	2988 bp	0.25	36.2
psbI_p2	CGATATATCGGACCCAATGGG	AACCAAGCAAAGCTTCTACGA	52.4	42.9	57.8	58.2	2989 bp	0.25	31.3
psbI_p3	CGATATATCGGACCCAATGGG	ACCAAGCAAAGCTTCTACGAA	52.4	42.9	57.8	58.2	2988 bp	0.25	31.3
psbI_p4	CGATATATCGGACCCAATGGG	AACCAAGCAAAGCTTCTACG	52.4	45.0	57.8	56.6	2989 bp	0.25	29.8
psbI_p5	CGATATATCGGACCCAATGGG	AAACCAAGCAAAGCTTCTACG	52.4	42.9	57.8	57.4	2990 bp	0.25	27.6